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Because of the highly conserved nature of UCEs and their flanking sequence, we have found these probes work well across amniotes. We are constantly evaluating the utility of given probe sets and probe designs, in addition to expanding the number of UCE loci we are targeting.We have several larger probes sets in the works for fishes, and we are also working on optimizing probe sets based on their capture success, phylogenetic utility, etc. You can now buy this probe set direct from MYcroarray in the form of a capture kit.2007), suggesting that their role in the biology of the genome may be cryptic.You can identify UCEs in organismal genome sequences by aligning several genomes to each other, scanning the resulting genome alignments for areas of very high (95-100%) sequence conservation, and filtering on user-defined criteria, such as length (e.g., Bejerano et al. If you want to use these regions as genetic markers, it is best to remove UCEs that appear to be duplicates of one another which we loosely define as being in more than one spot within each genome that you aligned.As their name implies, ultraconserved elements (UCEs) are highly conserved regions of organismal genomes shared among evolutionary distant taxa - for instance, birds share many UCEs with humans.UCEs were first described in a wonderful manuscript by Gil Bejerano et al.

We have created several software packages and we're working on tutorials to help get you started. A Phylogenomic Perspective on the Radiation of Ray-Finned Fishes Based upon Targeted Sequencing of Ultraconserved Elements (UCEs). We do not derive any referall revenue from these companies, but we list each here to help interested labs get started. We have several larger probes sets in the works, and we are also working on optimizing probe sets based on their capture success, phylogenetic utility, etc. You can now buy each of these probe sets direct from MYcroarray in the form of a capture kit.

Capable of demultiplexing hundreds of sequence tagged libraries at once. Get splitaake » A program for automated cleaning of fastq files from sequencing.

Removes adapter contamination using scythe and trims reads for quality using sickle.

We designed probes from UCEs by including flanking sequence from medaka.

Because of the highly conserved nature of UCEs and their flanking sequence, we have found these probes work well across fishes.

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